Investigació Aplicada en Malalties Respiratòries

Research Lines

Animal Model

Objectives
  • To provide a “pure” animal model of pneumonia associated with mechanical ventilation due to Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus
  • To study the efficacy of new antibiotics and their pharmacokinetic/pharmacodynamic (PK/PD) relationships to the aforementioned microorganisms
  • To study nonantibiotic therapies in pneumonia
  • To study ventilator associated pneumonia (VAP)
Projects in Progress
  • Linezolid versus vancomycin in pneumonia due to multiresistant Staphylococcus aureus (MRSA)
  • Study of biofilm in endotracheal tubes
  • Study of different techniques for obtaining samples for microbiologic diagnosis of VAP
Milestones Achieved
  • Model validation
  • Efficacy of intravenous corticosteroids for VAP due to Pseudomonas aeruginosa
Future Challenges
  • Consolidation of the animal model as a reference standard for studies of VAP
    • Demonstration of the use of new antibiotics and new forms of administration
    • Study of new systems for preventing community-acquired pneumonia (CAP)
    • Study of new systems for airway management

Publications

  • Effects of glucocorticoids in ventilated piglets with severe pneumonia

    Sibila O, Luna C, Agustí C, Baquero S, Gando S, Rizzo Patrón J, García Morato J, Absi R, Famiglietti A, Vay Carlos A., Von Stecher F, Torres A

    Eur Respir J 2008; 32: 1–10

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  • Experimental severe Pseudomonas aeruginosa Pneumonia and Antibiotic Therapy in piglets receiving Mechanical Ventilation

    Luna C, Baquero S, Gando S, Risso J, García J, Sibila O, Absi R, Famiglietti A, Vay C, Von Stecher F, Goffredo D, Bruno D, Agustí C, Torres A.

    Chest, August 2007, 132 (2): 523-531.

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  • Experimental Pseudomonas aeruginosa pneumonia: evaluation of the associated inflammatory response

    Sibila O, Agustí C, Torres A, Baquero S, Gando S, Patrón JR, Morató JG, Goffredo DH, Bassi N, Luna CM

    Eur Respir J 2007; 30 (6): 1167-1172

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  • Hemosiderin deposits confounds tracking of iron-oxide labelled stem cells

    Rigol M, Solanes N, Roqué M, Farré J, Batlle M, Roura S, Bellera N, Prat-Vidal C, Sionis A, Ramírez J, Sitges M, Sanz G, Bayés-Genís A, Heras M.

    Transpl Proc 2008; 40: 3619-3622.

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  • Effects of Cyclosporine, Tacrolimus and Sirolimus on Vascular Changes related to immune response: an Experimental Study

    Rigol M, Solanes N, Sionis A, Gálvez C, Martorell J, Rojo I, Brunet M, Ramírez J, Roqué M, E, Roig E, Pérez-Villa F, Barquín L, Pomar JL, Sanz G, Heras M.

    J Heart Lung Transplant 2008; 27(4): 416-22.

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  • An experimental model of pneumonia induced by methicillin-resistant Staphylococcus aureus in ventilated piglets

    Martínez-Olondris P, Sibila O, Agustí C, Rigol M, Soy D, Esquinas C, Piñer R, Luque N, Guerrero L, Quera MÁ, Marco F, de la Bellacasa JP, Ramirez J, Torres A

    Eur Respir J. 2010 Oct;36(4):901-6. Epub 2010 Mar 29.

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  • Development and validation of a high performance liquid chromatography method to determine linezolid concentrations in pig pulmonary tissue

    Guerrero L, Martínez-Olondris P, Rigol M, Esperatti M, Esquinas C, Luque N, Piñer R, Torres A, Soy D

    Clin Chem Lab Med. 2010 Mar;48(3):391-8

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  • Effects of adipose tissue-derived stem cells therapy after myocardial infarction. Impact of the route of administration

    Rigol M, Solanes N, Farré J, Roura S, Roqué M, Berruezo A, Bellera N, Novensà L, Tamborero D, Prat-Vidal C, Huzman M, Batlle M, Hoefsloot M, Sitges M, Ramírez J, Merino A, Sanz G, Brugada J, Bayés-Genís A, Heras M.

    J Cardiac Fail 2010;16:357-366.

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  • Efficacy of linezolid compared to vancomycin in an experimental model of pneumonia induced by methicillin-resistant Staphylococcus aureus in ventilated pigs

    Martinez-Olondris P, Rigol M, Soy D, Guerrero L, Agusti C, Quera MA, Li Bassi G, Esperatti M, Luque N, Liapikou M, Filella X, Marco F, de la Bellacasa JP, Torres A.

    Crit Care Med. 2011 Sep 15.

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  • An In Vitro Study to Assess Determinant Features Associated With Fluid Sealing in the Design of Endotracheal Tube Cuffs and Exerted Tracheal Pressures*    

    Li Bassi G, Ranzani OT, Marti JD, Giunta V, Luque N, Isetta V, Ferrer M, Farre R, Pimentel GL, Torres A.

    Crit Care Med. 2013 Feb;41(2):518-526.

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  • Effects of Manual Rib Cage Compressions on Expiratory Flow and Mucus Clearance During Mechanical Ventilation.    

    Martí JD, Bassi GL, Rigol M, Saucedo L, Ranzani OT, Esperatti M, Luque N, Ferrer M, Vilaro J, Kolobow T, Torres A.

    Crit Care Med. 2013 Jan 10.

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  • Linezolid limits burden of methicillin-resistant Staphylococcus aureus in biofilm of tracheal tubes.  

    Fernández-Barat L, Ferrer M, Sierra JM, Soy D, Guerrero L, Vila J, Li Bassi G, Cortadellas N, Martínez-Olondris P, Rigol M, Esperatti M, Luque N, Saucedo LM, Agustí C, Torres A.  

    Crit Care Med. 2012 Aug;40(8):2385-9.

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  • Direct analysis of bacterial viability in endotracheal tube biofilm from a pig model of methicillin-resistant Staphylococcus aureus pneumonia following antimicrobial therapy.  

    Fernández-Barat L, Li Bassi G, Ferrer M, Bosch A, Calvo M, Vila J, Gabarrús A, Martínez-Olondris P, Rigol M, Esperatti M, Luque N, Torres A.  

    FEMS Immunol Med Microbiol. 2012 Jul;65(2):309-17

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  • Effects of duty cycle and positive end-expiratory pressure on mucus clearance during mechanical ventilation*.  

    Li Bassi G, Saucedo L, Marti JD, Rigol M, Esperatti M, Luque N, Ferrer M, Gabarrus A, Fernandez L, Kolobow T, Torres A.  

    Crit Care Med. 2012 Mar;40(3):895-902.

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