Animal Model
Objectives
- To provide a “pure” animal model of pneumonia associated with mechanical ventilation due to Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus
- To study the efficacy of new antibiotics and their pharmacokinetic/pharmacodynamic (PK/PD) relationships to the aforementioned microorganisms
- To study nonantibiotic therapies in pneumonia
- To study ventilator associated pneumonia (VAP)
Projects in Progress
- Linezolid versus telavancin in pneumonia due to multiresistant Staphylococcus aureus (MRSA)
- Effect of Aerosolized antimicrobials in Pneumonia
- Study of monoclonal antibodies to combat bacterial infections
- Effect of corticosteroids in combination with antimicrobials to combat Streptococcus pneumoniae pneumonia
Milestones Achieved
- Model validation
- Efficacy of intravenous corticosteroids for VAP due to Pseudomonas aeruginosa
Future Challenges
- Consolidation of the animal model as a reference standard for studies of VAP
- Demonstration of the use of new antibiotics and new forms of administration
- Study of new systems for preventing community-acquired pneumonia (CAP)
- Study of new systems for airway management
Publications
Effects of glucocorticoids in ventilated piglets with severe pneumonia
Sibila O, Luna C, Agustí C, Baquero S, Gando S, Rizzo Patrón J, García Morato J, Absi R, Famiglietti A, Vay Carlos A., Von Stecher F, Torres A
Eur Respir J 2008; 32: 1–10
Experimental severe Pseudomonas aeruginosa Pneumonia and Antibiotic Therapy in piglets receiving Mechanical Ventilation
Luna C, Baquero S, Gando S, Risso J, García J, Sibila O, Absi R, Famiglietti A, Vay C, Von Stecher F, Goffredo D, Bruno D, Agustí C, Torres A.
Chest, August 2007, 132 (2): 523-531.
Experimental Pseudomonas aeruginosa pneumonia: evaluation of the associated inflammatory response
Sibila O, Agustí C, Torres A, Baquero S, Gando S, Patrón JR, Morató JG, Goffredo DH, Bassi N, Luna CM
Eur Respir J 2007; 30 (6): 1167-1172
Hemosiderin deposits confounds tracking of iron-oxide labelled stem cells
Rigol M, Solanes N, Roqué M, Farré J, Batlle M, Roura S, Bellera N, Prat-Vidal C, Sionis A, Ramírez J, Sitges M, Sanz G, Bayés-Genís A, Heras M.
Transpl Proc 2008; 40: 3619-3622.
Effects of Cyclosporine, Tacrolimus and Sirolimus on Vascular Changes related to immune response: an Experimental Study
Rigol M, Solanes N, Sionis A, Gálvez C, Martorell J, Rojo I, Brunet M, Ramírez J, Roqué M, E, Roig E, Pérez-Villa F, Barquín L, Pomar JL, Sanz G, Heras M.
J Heart Lung Transplant 2008; 27(4): 416-22.
An experimental model of pneumonia induced by methicillin-resistant Staphylococcus aureus in ventilated piglets
Martínez-Olondris P, Sibila O, Agustí C, Rigol M, Soy D, Esquinas C, Piñer R, Luque N, Guerrero L, Quera MÁ, Marco F, de la Bellacasa JP, Ramirez J, Torres A
Eur Respir J. 2010 Oct;36(4):901-6. Epub 2010 Mar 29.
Development and validation of a high performance liquid chromatography method to determine linezolid concentrations in pig pulmonary tissue
Guerrero L, Martínez-Olondris P, Rigol M, Esperatti M, Esquinas C, Luque N, Piñer R, Torres A, Soy D
Clin Chem Lab Med. 2010 Mar;48(3):391-8
Effects of adipose tissue-derived stem cells therapy after myocardial infarction. Impact of the route of administration
Rigol M, Solanes N, Farré J, Roura S, Roqué M, Berruezo A, Bellera N, Novensà L, Tamborero D, Prat-Vidal C, Huzman M, Batlle M, Hoefsloot M, Sitges M, Ramírez J, Merino A, Sanz G, Brugada J, Bayés-Genís A, Heras M.
J Cardiac Fail 2010;16:357-366.
Efficacy of linezolid compared to vancomycin in an experimental model of pneumonia induced by methicillin-resistant Staphylococcus aureus in ventilated pigs
Martinez-Olondris P, Rigol M, Soy D, Guerrero L, Agusti C, Quera MA, Li Bassi G, Esperatti M, Luque N, Liapikou M, Filella X, Marco F, de la Bellacasa JP, Torres A.
Crit Care Med. 2011 Sep 15.
An In Vitro Study to Assess Determinant Features Associated With Fluid Sealing in the Design of Endotracheal Tube Cuffs and Exerted Tracheal Pressures*
Li Bassi G, Ranzani OT, Marti JD, Giunta V, Luque N, Isetta V, Ferrer M, Farre R, Pimentel GL, Torres A.
Crit Care Med. 2013 Feb;41(2):518-526.
Effects of Manual Rib Cage Compressions on Expiratory Flow and Mucus Clearance During Mechanical Ventilation.
Martí JD, Bassi GL, Rigol M, Saucedo L, Ranzani OT, Esperatti M, Luque N, Ferrer M, Vilaro J, Kolobow T, Torres A.
Crit Care Med. 2013 Jan 10.
Linezolid limits burden of methicillin-resistant Staphylococcus aureus in biofilm of tracheal tubes.
Fernández-Barat L, Ferrer M, Sierra JM, Soy D, Guerrero L, Vila J, Li Bassi G, Cortadellas N, Martínez-Olondris P, Rigol M, Esperatti M, Luque N, Saucedo LM, Agustí C, Torres A.
Crit Care Med. 2012 Aug;40(8):2385-9.
Direct analysis of bacterial viability in endotracheal tube biofilm from a pig model of methicillin-resistant Staphylococcus aureus pneumonia following antimicrobial therapy.
Fernández-Barat L, Li Bassi G, Ferrer M, Bosch A, Calvo M, Vila J, Gabarrús A, Martínez-Olondris P, Rigol M, Esperatti M, Luque N, Torres A.
FEMS Immunol Med Microbiol. 2012 Jul;65(2):309-17
Effects of duty cycle and positive end-expiratory pressure on mucus clearance during mechanical ventilation*.
Li Bassi G, Saucedo L, Marti JD, Rigol M, Esperatti M, Luque N, Ferrer M, Gabarrus A, Fernandez L, Kolobow T, Torres A.
Crit Care Med. 2012 Mar;40(3):895-902.
Biofilms in ventilator-associated pneumonia.
Fernández-Barat L, Torres A
Future Microbiol. 2016 Dec;11:1599-1610. Epub 2016 Nov 10.
Lines list
- Animal Model
- Bronchiectasis non associated to Cystic Fibrosis (BQ-noFQ), Cystic Fibrosis (CF) and immune deficiencies
- Community-Acquired Pneumonia (CAP)
- Exacerbations of Chronic Obstructive Pulmonary Disease (COPD)
- Networks, European Projects and Collaborations
- Noninvasive Ventilation (NIV)
- Pulmonary complications in immunocompromised (IC) patients
- Translational research laboratory for advanced microbiology diagnosis and pathogen-host response in respiratory infections
- Ventilator-Associated Pneumonia (VAP)
